Protein Assays
American Research Products Inc Mouse L lactate dehydrogenase
Mouse L-lactate dehydrogenase A chain (LDH-A) ELISA Kit from CUSABIO Detection Range 0 156 mU/mL-10 mU/mL Sensitivity 0 039 mU/mL Sample serum plasma tissue homogenates Method Competitive
Sigma Aldrich Fine Chemicals Biosciences Lactate Dehydrogenase Inhi
A cell-permeable quinolinesulfonamide compound that acts as potent and reversible inhibitor of lactate dehydrogenase A (IC50 2.6 nM for human LDH-A). The inhibition appears to be competitive with respect to NADH and non-competitive with respect to pyruvate. Exhibits high selectivity over LDH-B (IC50 43 nM) and does not affect the activity of 32 other enzymes receptors and ion-channels even at high concentration (~ 10 181M). Rapidly reduces lactate production in hepatocellular (EC50 400 and 588 nM in Snu398 and HepG2 cells respectively) and breast carcinoma cell lines and induces PKM2 activation (EC50 600 nM in Snu398 cells). Shown to increase mitochondrial oxygen consumption rate (EC50 500 and 900 nM in Snu398 and HepG2 cells respectively) and causes a diminution in extracellular acidification rates (EC50 600 nM in both cell lines). Shown to downregulate the ability of A549 cells to form tumorspheres and diminishes the number of CD24/CD44-positive cells in HMLER system and i
Vector Biolabs Pyruvate dehydrogenase kinase, isozyme 2 Adenovirus (Ad-PDK2)
Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. Phospho-fructose kinase-2/fructose 2,6-bisphosphatase (PFK-2) stimulates the synthesis and degradation of fructose 2,6-bisphosphate. Pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate to generate acetyl-coenzyme A, thereby linking glycolysis to the TCA cycle with respect to substrate. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. TIM (Triosephosphate isomerase) exists as a homodimer and catalyzes the reaction of D-glyceraldehyde 3-phosphate into glycerone phosphate.
Bioassay Systems QuantiFluo™ HRP Reagent. Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA.
Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/585nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm). Key Features: For both absorbance or fluorescence detection. Highly sensitive. Convenient and fast. Method: OD570nm, FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests.
Revvity Health Sciences Inc HTRF EPIgeneous Binding Domain A Detection Kit, 500 Assay Points
HTRF EPIgeneous Binding Domain A Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF EPIgeneous Epigenetics Lysis Buffer A, 10,000 Assay Points
HTRF EPIgeneous Epigenetics Lysis Buffer A, 10,000 Assay Points
Revvity Health Sciences Inc HTRF EPIgeneous Epigenetics Lysis Buffer A, 500 Assay Points
HTRF EPIgeneous Epigenetics Lysis Buffer A, 500 Assay Points
Meso Scale Discovery U-PLEX Human DPPIV ASY384-well
The Meso Scale Discovery U-PLEX Human DPP4 384-Well Assay Kit is a 384-well electrochemiluminescent immunoassay for the quantitative measurement of DPP4 in human serum plasma and cell culture supernatants The assay uses MSD GOLD SULFO-TAG labels and MSD high bind plates to provide a simple no-wash protocol with high sensitivity and broad dynamic range The kit includes a 384-well plate coated with capture antibody detection antibody labeled with MSD GOLD SULFO-TAG calibrators and diluent The assay has a typical sensitivity of 0 08 ng/mL and an upper limit of quantification of 2500 ng/mL
Revvity Health Sciences Inc HTRF Cereblon Binding Kit, 10,000 Assay Points
HTRF Cereblon Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF EPIgeneous 2-methyl K36 Histone H3 Detection Kit, 10,000 Assay Points
HTRF EPIgeneous 2-methyl K36 Histone H3 Detection Kit, 10,000 Assay Points
Epigentek Inc Epigenase JMJD3/UTX Demethylase Activity/Inhibition Assay Kit (Colorimetric) (48 assays)
The Epigenase JMJD3/UTX Demethylase Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized reagents, designed for measuring activity/inhibition of JMJD3 and UTX using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.
Revvity Health Sciences Inc HTRF EPIgeneous Binding Domain B Detection Kit, 500 Assay Points
HTRF EPIgeneous Binding Domain B Detection Kit, 500 Assay Points
AURORA BIOLABS LLC TEV Protease Activity Assay, 96 rxn
Tobacco Etch Virus protease (TEV protease) is a highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. The kit contains a TEV protease substrate that is labeled with fluorophore FAM and a quencher. Proteolytic activity of TEV protease cleaves the substrate and releases the FAM, resulting in the production of bright fluorescence which can be measured using a fluorescence reader at ex/em of 490 nM/520 nm. TEV protease activity then can be calculated in accordance with the fluorescence intensity. Purified TEV protease is included in the kit as a positive control.
ABclonal Technology Lactate Dehydrogenase C
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis LDHC is testis-specific and belongs to the lactate dehydrogenase family Two transcript variants have been detected which differ in the 5 untranslated region
Biotium Protease Substrates Enzyme Substrates Protease Substrates Chemical Reagents 1/EA
This kit contains recombinant TEV Protease and Control Substrate both expressed from E coli along with 10X Reaction Buffer All kit components are supplied in solution and can be used to cleave affinity tags from fusion proteins using the TEV protease recognition site Unit size 1000 U